Protein & Gene Expression Analysis

At Sentebiolab, we provide protein and gene expression analyses for your research needs. We offer Western blotting, ELISA and qRT-PCR analyses as well as design and synthesis of primers for qRT-PCR and antibody-conjugation services.

Western Blotting

Western Blotting

For our western blot services, we use BioRad systems for vertical gel electrophoresis, semi-dry and wet transfer, imaging and documentation. Routinely used SDS-PAGE gels are produced in our lab. If desired, ready gels can also be used.

With Western blot, a host of applications are possible. For example, you can check the expression level of a protein of interest in cell lysates. Furthermore, the purity and identity of an expressed and purified protein can be checked.

Also, using Western blot, the presence and amount of expected antibodies in serum can be measured. The specific antibodies to be utilized in the Western blot procedure can be provided by Sentebiolab or by the customer as well. Our Western blot services also include a detailed report with analysis/discussion of the results.



qRT-PCR Analyses

We provide DNA/RNA isolation services, cDNA synthesis and gene expression analyses by using RT-PCR and qRT-PCR methodologies for research purposes. We can also design and optimize primers for these purposes.

ELISA

ELISA

We provide both direct and indirect ELISA services. We have experience with protocol optimization and statistical analysis of the results. We are capable of both performing ELISA using commercial kits and developing and optimizing custom assays. We provide a technical analysis result report together with the results of the service.

With this service, standard curve generation, antibody titration, cytokine/chemokine or the concentration measurement of a desired protein, Monte Carlo analysis for cut-off determination and implementation of variable statistical methods for sensitivity and specificity calculations is included.