DNA Probes

DNA probes are used in in situ techniques to determine whether or not a target sequence is present by hybridization with the target DNA or RNA sequence. The fluorescent markers found on these hybridized probes are what allow them to be visualized. This method is used to identify microorganisms and diagnose infectious, genetic and other diseases. Sentebiolab synthesizes molecular probes that you can use in your molecular biology research. The manufactured probes are subjected to quality control tests. We are able to modify probes with the following markers:

  • FAM-BHQ1
  • FAM-TAMRA
  • HEX-BHQ1
  • JOE-BHQ1

Probe purification methods:
  • This section is designed to help you choose the purification method most suitable for your application.
  • During oligo synthesis, every nucleotide is added to the growing chain successively.
  • In each assembly cycle, some nucleotides cannot be added to a small fraction of the oligo chains. This results in the production of a mixture of both full-length and truncated sequences.
  • During oligo synthesis, the separation of support and removal of protecting groups results in residual groups, which are removed by purification.
  • All probes are purified by HPLC.