qRT PCR Coronavirus Detection Kit

SENTEBIOLAB SENTELIGO CORONAVIRUS DETECTION KIT

What is SENTEBIOLAB Senteligo Coronavirus Detection Kit?

Senteligo coronavirus detection kit, developed by  Sentebiolab Biotechnology Ltd. Sti., has the ability to give results within 1 hour after RNA isolation. It is specially designed to diagnose SARS-CoV-2 virus, which causes COVID-19 disease. Our coronavirus detection kit eliminates the problems (primary-dimer, probe-dimer formation) associated with the kit developed by CDC, and is currently the most accurate kit on the market. It contains primer-probe sets that recognize 3 different regions in the N gene of the SARS-CoV-2 virus in addition to the positive control primer-probe set and the positive control DNA sample.

What is Coronavirus ?

Coronaviruses are a group of viruses that cause diseases in mammals and birds. Although the pathologies they create in humans are usually in the form of mild respiratory infections, several coronavirus types cause severe pathologies such as SARS, MERS, and COVID-19.

Coronaviruses   belong   to   the   Orthocoronavirinae   subfamily   of   the Cornidoviridae family in Nidovirales subgroup and are single-stranded RNA viruses.  They  are  known  by  the  envelope  structure  around  them  and contain a positive sense RNA sequence. Their genome lengths range from about 25 to 35 kb and carry crown-like proteins of about 20 nm in size.

The  SARS-CoV-2 virus,  which  was  first  seen  in  Wuhan in  2019,  is the causative agent of COVID-19 which has spread all over the world. SARS-CoV-

2 is a coronavirus similar to SARS-CoV and COVID-19 manifests by high fever, shortness of breath and dry cough in infected patients. The mortality rate of this disease increases with age, and it is observed to be more severe in men and especially in smokers and those with chronic diseases. COVID-19 infection turns into pneumonia when it is severe.

How to take samples for this test?

The World Health Organization (WHO) recommends RT-PCR-based testing to establish a definitive diagnosis in cases where COVID-19 infection is suspected. It is recommended that the samples to be taken for this test are taken from the respiratory tract (mouth swab, nose swab, or mucus sample (if any), etc.).

How should the samples be transferred?

After taking the samples, it is very important to keep them in the cold chain. In the cases where the test laboratories can be reached immediately, the samples can be kept at +4 °C, in cases where it will take a long time to reach the test centers, the samples should be frozen at –20 °C or preferably at -70 °C and kept in dry ice.

What types of tests can be done for the diagnosis of coronavirus?

In  order  to  conduct  molecular  tests,  the  relevant  laboratories  must  meet  the  biosecurity requirements at least at the BSL-2 level. After the swabs arrive in the laboratory, RNA should be isolated from the samples. It is not recommended to use heat-treatment prior to RNA isolation. WHO has published qRT-PCR methods developed by China, Germany, Hong Kong, Japan, Thailand, USA, and France on its website and stated that one of them can be used for SARS-CoV-2 diagnosis.

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Coronavirus Detection Kit